Abstract
Purpose
– To determine if the LifeWave X39 non-transdermal patch can produce changes in tripeptides
GHK and GHK-Cu production.
– To determine if the LifeWave X39 non-transdermal patch can produce changes in tripeptides
GHK and GHK-Cu production.
Materials
– BD Vacutainer Safety Loc Blood Collection sets with Pre-attached holder sized 21GX0.75 or
23GX0.75, lavender top tubes, KendroSorvallBiofuge Centrifuge 75005184+, sterile eye droppers,
polystyrene containers, thermal liners and an AB Sciex API4000 Qtrap. Analysis software included:
Qtrap Analyst software 1.6.2. and R software version 3.5.1.
– BD Vacutainer Safety Loc Blood Collection sets with Pre-attached holder sized 21GX0.75 or
23GX0.75, lavender top tubes, KendroSorvallBiofuge Centrifuge 75005184+, sterile eye droppers,
polystyrene containers, thermal liners and an AB Sciex API4000 Qtrap. Analysis software included:
Qtrap Analyst software 1.6.2. and R software version 3.5.1.
Method
– Blood was drawn into lavender top tubes on day 1, 2 and 7 with blood collection sets and
spun in KendroSorvall centrifuge for 10 minutes at1300rcf at room temperature. The plasma was
placed in cryo tubes and flash frozen using dryice, then shipped to the laboratory for analysis. Once
there the blood samples were processed according to the original thesis of Dr. Pickard. The filtrate was
– Blood was drawn into lavender top tubes on day 1, 2 and 7 with blood collection sets and
spun in KendroSorvall centrifuge for 10 minutes at1300rcf at room temperature. The plasma was
placed in cryo tubes and flash frozen using dryice, then shipped to the laboratory for analysis. Once
there the blood samples were processed according to the original thesis of Dr. Pickard. The filtrate was
concentrated by speed-vac and reconstituted with de-ionized water to50land analyzed with AB Sciex
API4000 Qtrap. The data was analyzed with Analyst software 1.6.2. Results were then sent for
statistical analysis using a Wilcoxon signed rank test. All reported p-values are two-sided and p< 0.05
was used to define statistical significance. Statistical analyses were conducted with R software version
API4000 Qtrap. The data was analyzed with Analyst software 1.6.2. Results were then sent for
statistical analysis using a Wilcoxon signed rank test. All reported p-values are two-sided and p< 0.05
was used to define statistical significance. Statistical analyses were conducted with R software version
3.5.1. “Copper tripeptide-1(GHK-Cu) is a small protein composed of the three amino acids (protein
building blocks) glycine, histidine, and lysine combined in a specific geometric configuration with the
physiologically beneficial mineral (copper) (DeHaven, 2014). It helps repair and maintain all tissue
types(DeHaven, 2014).
building blocks) glycine, histidine, and lysine combined in a specific geometric configuration with the
physiologically beneficial mineral (copper) (DeHaven, 2014). It helps repair and maintain all tissue
types(DeHaven, 2014).
Results
– There was a significant increase in GHK in blood seen at 24 hours, p< 0.0098. A significant
increase in GHK-Cu in the blood was also seen at 7 days, p< 0.0137.
– There was a significant increase in GHK in blood seen at 24 hours, p< 0.0098. A significant
increase in GHK-Cu in the blood was also seen at 7 days, p< 0.0137.
Conclusion
– This pilot study explored changes in amounts of GHK and GHK-Cu present inblood due
to wearing the LifeWave X39 patch for 1 week. There was a significant increase of GHK in blood seen
at 24 hours, p< 0.0098. A significant increase in GHK-Cu in blood was also seen at 7 days, p< 0.0137.
– This pilot study explored changes in amounts of GHK and GHK-Cu present inblood due
to wearing the LifeWave X39 patch for 1 week. There was a significant increase of GHK in blood seen
at 24 hours, p< 0.0098. A significant increase in GHK-Cu in blood was also seen at 7 days, p< 0.0137.